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. 2019 Oct;7(20):539. doi: 10.21037/atm.2019.10.07

Figure 5.

Figure 5

Reversed the activation of ASK1-JNK pathway can attenuate ES-induced apoptosis and JNK plays a vital role in autophagy induced by ES. (A,B) Pretreated with GS-4997 (GS; 1 µmol/L) and SP600125 (SP; 20 µmol/L) for 1 h, apoptosis was analyzed by FACS using an Annexin V-FITC/PI cell apoptosis kit; (C,D) pretreated with GS-4997 (GS; 1 µmol/L) and SP600125 (SP; 20 µmol/L) for 1 h, expression levels of indicated proteins were detected by western blot assay. All data are expressed as mean ± SD. **, P<0.01; ***, P<0.001 compared to the control group. ASK1, Apoptosis signal-regulating kinase 1; JNK, c-Jun N-terminal kinase; ES, Ecliptasaponin A; FACS, fluorescence-activated cell sorting; PI, propidium iodide.