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. 2019 Oct;7(20):561. doi: 10.21037/atm.2019.09.45

Figure 1.

Figure 1

Effects of Ang II on the the proliferation and migration of rat VSMCs at different time points. (A) Western blotting showed increased PCNA and OPN, and decreased α-SMA expressions mediated by Ang II at 12, 24 and 48 h (n=3). (B) CCK8 showed increased cell proliferation rate mediated by Ang II at 12, 24 and 48 h (n=5). (C) Transwell results showed increased VSMC migration mediated by Ang II at 12, 24 and 48 h (n=5). Scale bar: 200 µm. *P<0.05 vs. control, **P<0.01 vs. control, ***P<0.001 vs. control, #P<0.05 vs. Ang II 12 h, ##P<0.01 vs. Ang II 12 h, ###P<0.001 vs. Ang II 12 h, &P<0.05 vs. Ang II 24 h. Con, control group; Ang II, angiotensin II group; VSMC, vascular smooth muscle cell; PCNA, proliferating cell nuclear antigen; OPN, osteopontin; α-SMA, α-smooth muscle actin; CCK8, cell counting kit-8.