Figure 3.

Detection of mRNA expression levels by RT-qPCR (n=6 each group). Relative mRNA expressions of (A) Akt, (B) Bcl-2, and (C) Beclin 1 in the ventricular tissues of rats with sham operation (Sham), myocardial ischemia reperfusion (MIR), moxibustion pretreatment plus MIRI (Moxi), and autophagy inhibitor plus MIRI (3-MA). All data are expressed as mean ± SD. **, P<0.01 vs. Sham group; ^#, P<0.05 vs. MIR group; ^##, P<0.01 vs. MIR group. RT-qPCR, quantitative real-time polymerase chain reaction; MIRI, myocardial ischemia reperfusion injury.