Figure 3.

Viral loads of CSFV vary with the degree of injury in infected organs. About 100 mg tissues as indicated from the experimental pigs were collected for extraction of total RNA, 1 μg total RNA was transcribed into cDNA as template for absolute quantification of CSFV NS5B gene copies through real-time RT-PCR. A recombinant plasmid pMD18-T-NS5B was used as standard samples. The standard curve was constructed using 10-fold serially diluted standard plasmids. A linear regression plot was generated using GraphPad Prism 6 software (GraphPad Software Inc., San Diego, Calif). The slope, the Y-intercept and the R² value were determined (A). The viral loads in terms of NS5B copies/g tissues from the experimental pigs (B–D) were determined using the linear regression plot. Each template was ran in triplicate.