Table 1.
Real-time PCR quantification of Stenotrophomonas maltophilia on 16 sputa obtained from CF airways, with concurrent culture diagnosis
|
Sample |
ΔC t* |
Culture result |
Other known culture growth |
|---|---|---|---|
|
S. maltophilia isolate control |
2.5 |
Positive |
Pure isolate |
|
SCHI0002 day 1 |
7.9 |
Positive |
Mucoid and non-mucoid P. aeruginosa |
|
SCHI0002 day 320 |
6.0 |
Positive |
Mucoid P. aeruginosa |
|
SCHI0008 |
na |
nd |
nd |
|
SCHI0010 |
na |
Negative |
Mucoid and non-mucoid P. aeruginosa |
|
SCHI0011 |
12.4 |
nd |
nd |
|
SCHI0013 |
9.7 |
nd |
nd |
|
SCHI0014 |
8.0 |
Positive |
No other bacterial growth |
|
SCHI0019 day 1 |
6.7 |
Positive |
|
|
SCHI0019 day 11 |
2.5 |
nd |
nd |
|
SCHI0019 day 46 |
6.5 |
nd |
nd |
|
SCHI0020 day 1 |
17.0 |
Negative |
Mucoid P. aeruginosa |
|
SCHI0020 day 6 |
na |
nd |
nd |
|
SCHI0020 day 31 |
na |
nd |
nd |
|
SCHI0021 day 1 |
13.8 |
Negative |
MRSA, mucoid and non-mucoid P. aeruginosa |
|
SCHI0021 day 5 |
na |
nd |
nd |
|
SCHI0021 day 13 |
na |
nd |
nd |
MRSA, Methicillin-resistant Staphylococcus aureus; na, no amplification; nd, not determined.
*Cycles-to-threshold difference.