Figure 2.

MMP-9 is a target gene of miR-133a-3p. (A) TargetScan predicted the binding site between the MMP-9 3′-UTR and miR-133a-3p. (B) miR-133a-3p mimics or mimic controls and WT or MUT MMP-9 3′UTR were co-transfected into human vascular smooth muscle cells. Luciferase activity was detected using dual-luciferase reporter assay. Data are presented as the mean ± standard deviation. **P<0.01 vs. mimic control. MMP, matrix metalloprotease; miR, microRNA; UTR, untranslated region; WT, wild-type; MUT, mutant; has, homo sapiens.