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. 2019 Oct 1;18(6):4356–4362. doi: 10.3892/etm.2019.8070

Figure 5.

Figure 5.

Effect of miR-133a-3p on hVSMCs. hVSMCs were transfected with mimic control, miR-133a-3p mimic or miR-133a-3p mimic+MMP-9 plasmid for 48 h. (A) RT-qPCR detected levels of miR-133a-3p in hVSMCs. (B) RT-qPCR and (C) western blot analysis detected the mRNA and protein expressions of MMP-9 in hVSMCs, respectively. A (D) Cell Counting kit-8 assay was performed to determine cell proliferation and (E) Flow cytometry was used to detect cell apoptosis. Data are presented as the mean ± standard deviation. **P<0.01 vs. control; ##P<0.01 vs. mimic. miR, microRNA; hVSMCs, human vascular smooth muscle cells; MMP, matrix metalloprotease; RT-qPCR, reverse transcription-quantitative PCR; PI, propidium iodide.