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. 2019 Oct 15;18(6):4249–4258. doi: 10.3892/etm.2019.8103

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Copyright: © Shangguan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Knockdown of TOB1-AS1 induces proliferation of non-small cell lung cancer cells. (A) Analysis of the LncATLAS dataset indicated that TOB1-AS1 localized in the cytoplasm and nucleus in most of the cell lines. NEAT1 was used as a positive control of nuclear genes and H19 was used as a positive control of cytoplasmic genes. (B) Reverse transcription-quantitative PCR analysis indicated that TOB1-AS1 was located in the cytoplasm and nucleus of the A549 and H1299 cell lines. (C and D) Knockdown efficiency of TOB1-AS1 by using siRNA-1 and siRNA-2 in (C) A549 and (D) H1299 cells compared with NC. *P<0.05 and **P<0.01, vs. NC. (E and F) Knockdown of TOB1-AS1 induced cell proliferation in (E) A549 and (F) H1299 cells. ***P<0.001 vs. NC. TOB1-AS1, transducer of ERBB2, 1-antisense 1; NEAT1, nuclear paraspeckle assembly transcript 1; siRNA, small interfering RNA; NC, negative control; OD, optical density; Cyto, cytosol; CN RCI, Cytoplasmic/Nuclear concentration index (RCI).