Figure 1.

NS-SV-AC cells in EYP Biomaterial combinations analyzed at 0 and 14 days. In panels of biomaterial combinations, either EYP + Media or EYP + EW, images taken by microscopy show 25× (upper panel) and 200× (lower panel) magnification of the sagittal sections grown in 3D-Cryo well inset. Sirius Red Chemical Stain was used to stain sagittal sections. Biomaterials were in red/pink, while cells were in black. (A) Cells in EYP + Media 1:1 mixture; (B) Cells in EYP + EW 45:55 mixture. The word “band” highlights the approximate thickness of the sagged protein network in the EW at T = 14 d.