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. 2019 Oct 24;12(21):3480. doi: 10.3390/ma12213480

Figure 5.

Figure 5

3D-Printing of freeze-thawed GEYP and implementation of 3D-Cryo well insert for characterization of extruded structure. (A) Pneumatic bioprinter print head rapidly extruding cylinders of freeze thawed GEYP on the lid of a 96 well plate; (B) Various layers involved in printing CAD designed model to study interfaces (ball and socket); in this structure, no cells are involved; (C) 3D-printed ball and socket model sectioned in half with glass coverslip to show sagittal/coronal plane (top left). Manually extruded reproduction of ball and socket model in histology well insert loaded with various fluorescent labelled cells (centre left). 3D-Cryo well insert mounted on cryotome cutting block sectioned to the core (bottom left). Examination of the resulting sagittal/coronal section from the 3D-Cryo well insert mounted on a glass slide and imaged with fluorescence light (Right). Cells are seen in green and blue distributed in the biomaterial. As they were extruded in separate inks, they are also separated in the images. White triangles show the interface line.