Table 2.
Gene expression of Nfe2l2 and Nrf2 target genes, nitric oxide synthase and cytokine-encoding genes at 32 h and 3 months post-TBI in the rat perilesional cortex.
| Gene | 32 H | 3 Months | ||
|---|---|---|---|---|
| Log2 FC | FDR | Log2 FC | FDR | |
| Nfe2l2 and Nrf2 Target Gene Expression | ||||
| Nfe2l2 | 0.094 | 6.41 × 10−1 | 0.601 | 6.41 × 10−5 |
| Gclm | 0.818 | 1.88 × 10−8 | 0.016 | 9.51 × 10−1 |
| Hmox1 | 2.860 | 1.69 × 10−9 | 1.189 | 2.69 × 10−18 |
| Nqo1 | 0.424 | 6.45 × 10−3 | 0.646 | 1.29 × 10−7 |
| Nitric Oxide Synthase Gene Expression | ||||
| Nos1 | 0.038 | 6.22 × 10−1 | 0.209 | 3.50 × 10−1 |
| Nos2 | −0.324 | 7.05 × 10−7 | 0.587 | 3.32 × 10−2 |
| Nos3 | 0.203 | 1.22 × 10−1 | −0.023 | 9.30 × 10−1 |
| Cytokine Gene Expression | ||||
| Il1a | −0.693 | 1.08 × 10−4 | −0.089 | 8.50 × 10−1 |
| Il1b | 0.478 | 6.15 × 10−2 | 0.598 | NA |
| Il2 | −0.314 | 6.57 × 10−6 | −0.009 | NA |
| Il4 | −0.177 | 1.38 × 10−4 | 0.095 | NA |
| Il5 | −0.149 | 9.87× 10−4 | NA | NA |
| Il6 | 0.409 | 2.62 × 10−2 | −0.013 | NA |
| Il10 | −0.300 | 3.81 × 10−3 | 0.161 | NA |
| Il12a | −0.409 | 5.12 × 10−5 | −0.002 | 9.96 × 10−1 |
| IL-13 | −0.247 | 1.39 × 10−3 | −0.046 | NA |
| Csf2 (GM-CSF) | −0.211 | 5.91 × 10−3 | NA | NA |
| Ifng | −0.414 | 2.19 × 10−2 | NA | NA |
| Tnf | −0.147 | 5.24 × 10−1 | 0.088 | 8.53 × 10−1 |
Abbreviations: FC, fold-change as compared injured to sham-operated experimental controls; FDR, false discovery rate; NA, no detectable expression or no p-value available. At 32 h post-TBI, 3 sham-operated and 3 injured controls were used. At 3 months post-TBI, 5 sham-operated and 5 injured rats were used. Statistical significance: FC and corresponding FDR (<0.05) are shown in bold font.