Table 3.
Target engagements measured as a gene expression of Nfe2l2, Gclm, Hmox1 and Nqo1 after 16-h treatment with desmethylclomipramine, ionomycin or sulforaphane under LPS/IFNy-induced inflammatory or non-inflammatory conditions in BV2-microglia cell cultures.
| Drug | Inflammatory (LPS/IFNy) | Non-Inflammatory (No LPS/IFNy) | ||
|---|---|---|---|---|
| Log2 FC | p-Value | Log2 FC | p-Value | |
| Desmethylclomipramine | ||||
| Nfe2l2 | 0.282 | 1.87 × 10−2 | 0.200 | 3.83 × 10−2 |
| Gclm | 0.099 | 7.70 × 10−1 | 0.118 | 7.83 × 10−1 |
| Hmox1 | 0.039 | 7.85 × 10−1 | 0.050 | 3.98 × 10−1 |
| Nqo1 | −0.056 | 6.64 × 10−1 | −12.419 | 1.09 × 10−1 |
| Ionomycin | ||||
| Nfe2l2 | −0.052 | 7.87 × 10−1 | 0.125 | 1.65 × 10−1 |
| Gclm | 0.262 | 1.04 × 10−1 | 0.388 | 4.52 × 10−1 |
| Hmox1 | 0.298 | 1.54 × 10-1 | 0.281 | 6.58 × 10-2 |
| Nqo1 | 0.565 | 8.72 × 10-3 | −12.283 | 8.89 × 10-2 |
| Sulforaphane | ||||
| Nfe2l2 | 0.723 | 3.01 × 10−7 | −0.382 | 1.26 × 10-2 |
| Gclm | 1.117 | 7.47 × 10−8 | 3.305 | 4.61 × 10−15 |
| Hmox1 | 0.171 | 1.52 × 10-2 | 1.403 | 3.14 × 10−9 |
| Nqo1 | 1.897 | 1.03 × 10−12 | −6.055 | 7.23 × 10-2 |
Abbreviations: FC, fold-change compared to untreated control; LPS, lipopolysaccharide, IFNγ, interferon gamma. The experiment was performed twice, each including quadruplicates. Statistical significances: FC-change and corresponding p-value (<0.05) from linear regression model are shown in bold font.