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. 2019 Oct 23;20(21):5263. doi: 10.3390/ijms20215263

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Enzymatic glycosylation for the production of N-acetyl-d-lactosamine from glucose-6-phosphate and N-acetyl-d-glucosamine (a) [208]. The Δ_rG′° represents the standard change in Gibbs free energy (b) was calculated using the eQuilibrator web interface (http://equilibrator.weizmann.ac.il) [155] using the following conditions: ionic strength 0.1 M, pH 7.0, 1 mM of component, 298 K. Abbreviations: Glc-6-P, d-glucose-6-phosphate; Glc-1-P, d-glucose-1-phosphate; P₂O₇⁴⁻, pyrophosphate; HPO₄²⁻, orthophosphate; UDP, uridine diphosphate; PGM, Phosphoglucomutase; UDPG-P, UDP-glucose pyrophosphorylase; GalE, UDP-galactose epimerase; GalT, galactosyltransferase; PK, pyruvate kinase.