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. 2019 Nov 3;20(21):5476. doi: 10.3390/ijms20215476

Figure 3.

Figure 3

Percentage of different testicular cell types in testicular cell suspension (TCS). (A) The graph represents the percentage of germ cells (GCs; DDX4), Sertoli cells (SCs; SOX9), Leydig cells (LCs; CYP19A1) and peritubular cells (ACTA2) in TCS obtained following digestion of ITT and used for testicular organoid (TO) generation. (B) Representative image of DDX4, SOX9, CYP19A1 and ACTA2 immunofluorescence analysis of TCS used for generation of TOs. n = 4.