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. 2019 Oct 24;20(21):5273. doi: 10.3390/ijms20215273

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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IL-6 activates the STAT3 signaling pathway in C2C12 cells. (A) Evaluation of p-STAT3 protein levels in C2C12 cells at 5 min up to 30 min after treatment of cells with low, medium and high IL-6 concentrations. (B) Quantification of western blot images shown in (A). (C) Effect of JAK-STAT inhibitor, AG490, on p-STAT3 expression in C2C12 cells over 15 min after treatment with low and high IL-6 concentration. (D) Effect of JAK-STAT inhibitor, AG490, on PCNA, MyoD and myogenin protein levels in C2C12 cells after treatment with low and high IL-6 concentrations (top panel) and densitometric quantification of images (lower panel) (E) Quantification of PCNA, MyoD and myogenin protein levels in C2C12 cells after treatment with low and high IL-6 concentrations plus JAK-STAT inhibitor, AG490. The experiment was repeated three times. Immunofluorescence images were taken at 100× magnification. Results are presented as mean ± standard error of the mean (S.E.M). Statistical analysis: ANOVA and Bonferroni post hoc test. * p < 0.05; ** p < 0.01; *** p < 0.001.

IL-6 activates the STAT3 signaling pathway in C2C12 cells. (A) Evaluation of p-STAT3 protein levels in C2C12 cells at 5 min up to 30 min after treatment of cells with low, medium and high IL-6 concentrations. (B) Quantification of western blot images shown in (A). (C) Effect of JAK-STAT inhibitor, AG490, on p-STAT3 expression in C2C12 cells over 15 min after treatment with low and high IL-6 concentration. (D) Effect of JAK-STAT inhibitor, AG490, on PCNA, MyoD and myogenin protein levels in C2C12 cells after treatment with low and high IL-6 concentrations (top panel) and densitometric quantification of images (lower panel) (E) Quantification of PCNA, MyoD and myogenin protein levels in C2C12 cells after treatment with low and high IL-6 concentrations plus JAK-STAT inhibitor, AG490. The experiment was repeated three times. Immunofluorescence images were taken at 100× magnification. Results are presented as mean ± standard error of the mean (S.E.M). Statistical analysis: ANOVA and Bonferroni post hoc test. * p < 0.05; ** p < 0.01; *** p < 0.001.