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. 2019 Oct 7;18(6):4231–4240. doi: 10.3892/etm.2019.8081

Figure 3.

Figure 3.

A2B expression profiles in liver cancer cell lines with gain- or loss- of HIF-1α function under hypoxic conditions. Western blot analyses of the expression of (A) HIF-1α and A2B and A2B transcript levels under hypoxic (0.5% oxygen) conditions for (B) up to 12 h in HepG2 cell lines. Cells were transfected with either scrambled-siRNA or HIF-1α-siRNA. Western blot analyses of (C) HIF-1α and A2B expression and A2B transcript levels under hypoxic (0.5% oxygen) conditions for (D) up to 12 h in HepG2 cell lines. Cells were transfected with either empty vector pcDNA3.1 (pcDNA-cont) or HIF-1α-pcDNA3.1 (pcDNA-HIF-1α) plasmid. (E) Cells were treated with different doses of echinomycin, a HIF-1α chemical inhibitor, for (F) up to 12 h. The expression of A2B transcript and protein levels were analyzed using RT-qPCR and western blot analysis, respectively. Data are presented as the mean ± standard deviation (n=4). Actin was used as a loading control housekeeping gene for use in western blot analysis. GAPDH was used as the housekeeping gene for RT-qPCR. A2B, adenosine A2B receptor; HIF-1α, hypoxia inducible factor-1α; siRNA, small-interfering RNA; RT-q, reverse transcription-quantitative.