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. 2019 Oct 7;18(6):4231–4240. doi: 10.3892/etm.2019.8081

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Copyright: © Kwon et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — siRNA-mediated A2B silencing inhibits HepG2 cell proliferation. (A) HepG2 cells were transfected with either scr-siRNA or A2B-siRNA. Transfection efficiency of A2B-siRNA was measured using western blot analysis. Actin was used as a loading control. (B) Cell proliferation was determined by measuring BrdU incorporation for up to 96 h (n=4). (C) Relative cell growth rates were examined by the MTT assay. (D) Cell viability of cells at each time-point was measured by MTT assay. Data are presented as the mean ± standard deviation (n=4). Cells transfected with scr-siRNA was used as control and P<0.05 was considered to indicate a statistically significant result. siRNA, small-interfering; A2B, adenosine A2B receptor; scr, scrambled.