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. 2019 Oct 7;18(6):4231–4240. doi: 10.3892/etm.2019.8081

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Copyright: © Kwon et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Protein levels of A2B and HIF-1α measured in human HCC specimens and in non-cancerous tissue specimens, and mRNA levels of A2B isoforms. (A) HIF-1α and A2B protein expression were detected using western blot analysis. Specimens from five patients (P1-P5) who underwent HCC hepatectomy and non-cancerous tissue specimens around the tumors. Actin was used as a loading control. Relative expression of (B) HIF-1α and (C) A2B by densitometric quantification of western blots. (D) mRNA levels of A2B and other adenosine isoform receptors from five patients. The expression levels are presented relative to those of the control housekeeping gene GAPDH. The results are presented as the mean ± standard deviation; P<0.05; Student's t-test. A2B, adenosine A2B receptor; HIF-1α, hypoxia inducible factor-1α; HCC, hepatocellular carcinoma; T, HCC hepatectomy; N, non-cancerous tissue specimens.