Skip to main content
. 2019 Oct 23;20(21):5248. doi: 10.3390/ijms20215248

Figure 1.

Figure 1

A rapamycin trap for evaluating the presence of nuclear envelope transmembrane proteins (NETs) on the INM: (a) A membrane bound NETs tagged with a fluorophore and FRB will dimerize with another fluorescently tagged protein with an 12 kDa FK506 binding protein (FKBP12) domain in the presence of rapamycin, represented here as a triangle. (b) In the absence of rapamycin, the soluble Green Fluorescent Protein (GFP) labeled protein (green blocks) will not dimerize with the membrane bound NETs (red blocks) and diffuse throughout the nucleus. (c) In the presence of rapamycin the soluble GFP labeled protein (green blocks) will dimerize with the membrane bound NETs (red blocks) and localize to the nuclear envelope.