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. 2019 Oct 25;20(21):5329. doi: 10.3390/ijms20215329

Figure 1.

Figure 1

Immunolocalization of A2A and A2B adenosine receptors in the pancreatic islets of the rat. (A) Fluorescence of A2A adenosine receptor in the cell membrane (arrowheads). (B) Fluorescence image of insulin. (C) Overlay image of A and B. (D) Overlay of A and fluorescence image of glucagon (orange) in the same sample. (E) Fluorescence of A2B adenosine receptor in cells (arrowheads). (F) Fluorescence image of insulin. (G) Overlay image of E and F. (H) Overlay of E and fluorescence image of glucagon (orange). (I) Isotype control image of the rat pancreas with normal rabbit IgG (2 μg/mL). (J) Fluorescence image of insulin. (K) Overlay image of I and J. (L) Overlay of I and fluorescence image of glucagon (orange). 4′,6-diamidino-2-phenylindole (DAPI) was used to stain nuclei (blue). Representative images of three rats are shown. Bars = 20 μm.