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. 2019 Oct 24;20(21):5299. doi: 10.3390/ijms20215299

Figure 2.

Figure 2

Phosphatase of regenerating liver-1 (PRL-1)-dependent migration ability under hypoxic conditions regulated by miRNAs targeting the integrin family.(A) Representative images and (B) the number of migrated naïve and PRL-1(+) PD-MSCs using a Transwell insert system under 1% hypoxic conditions for 24 h. (C) mRNA expression of hypoxia-inducible factor 1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) in migrated PRL-1(+) PD-MSCs determined using a Transwell insert system under 1% hypoxic conditions for 24 h, as determinedby qRT-PCR. (D) Ras homolog family member A (RHOA) and RHO-associated coiled-coil-containing protein kinase 1 (ROCK1) levelsin migratednaïve and PRL-1(+) PD-MSCs under 1% hypoxic conditions as determined by qRT-PCR. (E) mRNA expression levels of PRL-1 and targeted hsa-miR-30a-5p expression, (F) Integrin alpha 4 (ITGA4) and targeted hsa-miR-340-5p expression, and (G) integrin beta 7 (ITGB7) and targeted hsa-miR-146a-3p expression in migrated PRL-1(+) PD-MSCs under 1% hypoxic conditions for 24 h as determined by qRT-PCR. Luciferase assay of PRL-1, ITGA4, and ITGB7 containing hsa-miR-30a-5p, 340-5p, and 146a-3p binding sitesin (H) naïve and (I) PRL-1(+) PD-MSCs. Firefly luciferase activities were measured by luminescence. All experiments were conducted in at least triplicate. Data from each group are expressed as means ± SD, determined by Student’s t-test. Scale bars = 100 μm.* p < 0.05 vs. normoxia, # p < 0.05 vs. naïve, and ** p < 0.05 vs. negative control (NC).