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. 2019 Oct 24;20(21):5299. doi: 10.3390/ijms20215299

Figure 6.

Figure 6

miRNAs mediated hepatic regeneration by PRL-1(+) PD-MSCs in a rat model of BDL through interleukin-6 (IL-6)/signal transducer and activator of transcription 3 (STAT3) signaling. (A) qRT-PCR of rno-miR-21-5p-targeted interleukin-6 receptor (IL-6R) and (B) Western blotting of glycoprotein 130 (gp130), IL-6, and phosphorylated STAT3 levels in BDL-injured rat liver tissue following the administration of naïve and PRL-1(+) PD-MSCs at one, two, three, and fiveweeks. (C) mRNA expression of hepatocyte nuclear factor 4 alpha (HNF4A) and (D) miR-122-5p-targeted HNF1 homeobox A (HNF1A) in BDL-injured rat liver tissue by qRT-PCR. (E) Proliferating cell nuclear antigen (PCNA) expression in the rat liver sections from each group (Con, NTx, TTxNaïve, and TTxPRL-1(+)) at one week as determined by immunohistochemistry. (F) Quantification of the PCNA-positive area in hepatocytes. Scale bars = 50μm. All experiments wereconductedin at least triplicate.Data from each group are expressed as means ± SD, determined by Student’s t-test.* p < 0.05 vs. NTx and # p < 0.05 vs. TTx Naïve, wk: week.