Figure 1.

Structure, evolution, and expression of GarWRKY5. (a) Identification of the WRKY domain. The approximately 60-amino acid WRKY domain and C–X₇–CX₂₃–HXC-type zinc finger-like motif. The 60 amino acid WRKY domain was indicated by a red line and the conserved WRKYGQK sequence was black squares. The C and H residues of zinc finger domain were indicated by red triangles. (b) Phylogenetic analysis of GarWRKY5 between homologous genes from G. hirsutum (Gh_A02G0029/Gh_D02G0043), G. raimondii (Gorai.005G003900), and G. arboreum (Cotton_A_04316). (c) The expression of GarWRKY5 in different tissues and organs from G. aridum. The relative expression levels were calculated with three biological replicates and three experimental replicates. Standard deviation (SD) was used to calculate the error bars.