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. 2019 Oct 26;20(21):5334. doi: 10.3390/ijms20215334

Figure 2.

Figure 2

(A) C. arabica embryogenic calli induced from leaf explants used for stable genetic transformation. (B) Hygromycin-resistant embryogenic calli of C. arabica derived from bombarded globular structures after four months of selection, showing independent events of genetic transformation. (C) Somatic embryos under osmotic stress in the first month of maturation. (D,E) Individual somatic embryo under osmotic stress in the second month of maturation. Scale bar 1000 μm. (F) Green fluorescent protein (GFP) stable expression in nuclei of transgenic mature somatic embryos of C. arabica, after three months of growth on 50 mg/L hygromycin-containing medium. (G) Control somatic embryo, showing no GFP expression. (H) Regenerated plants of C. arabica after six months in soil conditions. Wild-type plants in red pots, transgenic plants in green and blue pots.