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. 2019 Oct 26;20(21):5334. doi: 10.3390/ijms20215334

Figure 4.

Figure 4

Southern blot analysis of transgenic coffee plants. (A) Southern blot hybridization analysis of regenerated plants of C. arabica digested with EcoR1. (B). The blot was hybridized with Biotin labeled cry10Aa 1996 bp fragment. Lanes E1, E2, E3, and E4 correspond to genomic DNA from three independent transformation events. C-lane: DNA extracted from an untransformed control plant.