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(A) SDS-PAGE of pre-purified proteins from plant tissues, M (molecular weight marker), C (wild-type plant), E2–E3–E4 (transformation events); (B) Western blot, putative Cry10Aa protein is indicated; (C) Mass spectrometry analysis from SDS-PAGE bands corresponding to the Cry10Aa protein (shadowed sequence fragments confirm the expression of Cry10Aa) with a coverage of 76% of the Cry10Aa protein.
