Table 1.
Summary of genetic transformation events of C. arabica mediated by particle bombardment of somatic embryos with plasmid pMDC85 under different osmotic conditions.
| Treatment | Bombarded Plates | Transgenic Embryogenic Lines | Transformation Efficiency (%) | Transformed Events/Plate | Plants Grown (%) |
|---|---|---|---|---|---|
| CP2 (control) | 50 | 85 | 8.5 | 1.7 | 98 |
| Sucrose 6% | 50 | 256 | 25.6 | 5.12 | 100 |
| Sucrose 12% | 50 | 133 | 13.3 | 2.56 | 87 |
| Mannitol 0.15 M + Sorbitol 0.15 M | 50 | 197 | 19.7 | 3.94 | 95 |
| Mannitol 0.3 M + Sorbitol 0.3 M | 50 | 164 | 16.4 | 3.28 | 80 |