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. 2019 Oct 30;20(21):5415. doi: 10.3390/ijms20215415

Figure 3.

Figure 3

Oxa up-regulates the expression of PFKFB3. (a) Following treatment with Oxa for 6 h, culture media were collected and subjected to lactate assay. (b) Real-time PCR was performed to detect the mRNA expression of PFKFB3 following treatment with Oxa for 6 h. (c–f) SW480 cells were treated with Oxa (e: 50 μM) upon to 2 h (c: 2 h). Cell lysates were subjected to immunoblotting with the indicated antibodies, and the relatively ratios of PFKFB3 and p-Ulk1 to Actin were shown in the histogram graphs. * p < 0.05 vs. control, and ** p < 0.01 vs. control. Data represent three independent experiments.