Figure 1.

Effect of callus induction from mature embryos in Bd21. (a). A photograph depicting a sheared seed, scale bars, 1 mm. (b and c). The callus induction from sheared seeds (b) and untreated seeds (c) on different days. The lower panels in (b) or (c) show the growth of ten seeds on CIM (callus induction medium)_petri dish (MS + 2.5 mg/L 2,4-D + 0.6 mg/L CuSO₄ + 3 g/L Phytagel), scale bars, 1 cm. The upper panel showed a close-up image of a single seed of the 10 seeds from the corresponding lower petri dish (red arrow), scale bars, 1 mm. For each treatment, three replicates (each of 10 seeds) were conducted.