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. 2019 Oct 28;20(21):5366. doi: 10.3390/ijms20215366

Figure 6.

Figure 6

Cordycepin and IR induce G2/M cell cycle arrest through autophagy induction in oral cancer cells. (a) Cell cycle distribution after treatment with 3-MA (1 mM) and shATG5 plus cordycepin (50 μM) and/or IR (4 Gy) for 6, 12, and 18 h in SAS cells. (b) Quantification of G2/M phase. Cells were pretreated with 3-MA (1 mM) followed by cordycepin (50 μM) and/or IR (4 Gy) for 6, 12, and 18 h. ** p < 0.01. (c) Quantification of G2/M phases. SAS cells were transfected with ATG5 shRNA and treated with cordycepin (50 μM) and/or IR (4 Gy) for 6, 12, and 18 h. * p < 0.05. (d) Western blot analysis of p53, p21, and p27 expression in the absence and presence of 3-MA. Cells were pretreated with 3-MA (1 mM) for 1 h before receiving the combined treatment (4 Gy IR and 50 μM cordycepin) for 24 h. (e) Western blot analysis of p53, p21, p27, and ATG5 protein expression in SAS cells transfected with ATG5 shRNA. shATG5 cells were treated with 4 Gy IR and/or 50 μM cordycepin for 24 h.