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TNBS– release after reaction of the rRNase with substoichiometric DTNB. (A) Fully reduced rRNase (1.25 µM, 10 µM protein –SH) was reacted with 1.25 µM DTNB at pH 5.0, 25 °C (red line); the rRNase (1.25 µM, 10 µM protein –SH) was reacted with 5.0 µM DTNB at pH 5.0, 25 °C (blue line). (B) Schematic representation of the reaction of the rRNase with stoichiometric DTNB.
