Skip to main content
. 2019 Oct 31;20(21):5440. doi: 10.3390/ijms20215440

Figure 6.

Figure 6

Circular dichroism spectra of the RNase and the effect of urea on the hyper-reactivity of rRNase cysteines. (A) CD spectra of native ribonuclease (black line); reduced RNase in 0.2 M urea (blue line); reduced RNase in 8 M urea (red line). CD spectra were recorded at pH 7.4 (see Materials and Methods). (B) CD spectra of alkylated rRNase (green line); glutathionylated/alkylated rRNase (magenta line). CD spectra were recorded in 0.2 M urea at pH 7.4 (see Materials and Methods). (C) Hyper-reactivity of rRNase cysteines (10 µM) toward DTNB (20 µM) at different urea concentrations (pH 5.0, 25 °C) (fit obtained using equation (5)). Average ± SD from three independent experiments.