Figure 9.

GLP-1 and pegGLP-1 treatment effects on CD31⁺ endothelial cells isolated from the lungs of male and female C57BL/6 mice in vitro: (a) Images of CD31⁺ cells stained with: Hoechst (blue) to identify cell nuclei; CD34 FITC (green) (Hoechst + CD34) composite image using all two colors. All scale bars are 100 µm; (b–g) CD31+ endothelial cells from lung were precultured for 5 days, incubated with or without GLP-1 (10⁻⁷ M) or pegGLP-1 (10⁻⁷ M) for 24 h and then labeled with Hoechst, Carboxyfluorescein succinimidyl ester (CFSE) (b,e) CD34 FITC (c,f), Annexin V and 7-Aminoactinomycin D (7-AAD) (d,g) prior to fluorescence microscopic analysis. (b) CFSE activity after culture of cells isolated from the lung of intact mice; (c) the level of CD34⁺ cells after culture of cells isolated from the lung of intact mice; (d) the count of cells with apoptosis after culture of cells isolated from the lung of intact mice; (e) CFSE activity after culture of cells isolated from the lung of mice with MD and lung emphysema; (f) the level of CD34⁺ cells after culture of cells isolated from the lung of mice with MD and lung emphysema; (g) the count of cells with apoptosis after culture of cells isolated from the lung of mice with MD and lung emphysema. All data are expressed as mean ± SD, *—significance of difference compared with control (p < 0.05).