Expression analyses of flavonoid biosynthetic pathway key genes in transgenic flowers carrying NtMYB3 by qPCR. L-2, L-4, indicated two transgenic tobacco lines. PAL, Phenylalanine ammonia lyase; 4CL, 4-coumaroyl-CoA ligase, CHS, Chalcone synthase; CHI, Chalcone isomerase; F3H, Flavanone 3-hydroxylase; DFR, Dihydroflavonol 4-reductase; FLS, flavonol synthase LAR, leucoanthocyanidin reductase; ANR, anthocyanidin reductase; ANS, anthocyanin synthase; UFGT, UDP glucose-flavonoid 3-O glucosyltransferase. Small letter showed the significant difference from wild type at the level of p < 0.05 by using LSD test. The bar represents the standard error of thrice biological replications.