Figure 3.

Effects of GLYX-13 on the phosphorylation and gene transcription levels of NR2B, CaMKII and CREB in the hippocampus of mice after isoflurane exposure.

(A–C) The phosphorylation levels of NR2B, CaMKII and CREB at 1 (A), 3 (B) and 7 (C) days after isoflurane anesthesia, detected by western blot assay. Relative protein expression is expressed as the ratio of the target protein to β-actin. (D–F) NR2B, CaMKII and CREB mRNA levels at 1 (D), 3 (E) and 7 (F) days after isoflurane anesthesia detected by real time-polymerase chain reaction. Values are expressed as the mean ± SEM (n = 5). *P < 0.05, vs. Con group; #P < 0.05, vs. Anes group (one-way analysis of variance followed by post-hoc least significant difference test). CaMKII: Ca²⁺/calmodulin-dependent protein kinase II; CREB: cyclic adenosine monophosphate response element binding protein; NR2B: N-methyl-D-aspartate receptor subunit 2B. Con: Control group; Anes: isoflurane anesthesia group, in which mice were exposed to 1.5% isoflurane for 6 hours; GLYX-13: GLYX-13 injection group, in which mice were intravenously administered GLYX-13 (1 mg/kg); Anes + GLYX-13: isoflurane anesthesia + GLYX-13 injection group, in which mice were exposed to 1.5% isoflurane for 6 hours and intravenously pre-administered GLYX-13 (1 mg/kg) 2 hours earlier.