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. 2019 Oct 24;20(21):5281. doi: 10.3390/ijms20215281

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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HAGG suppressed GEC tube formation on Matrigel. GECs were seeded on growth factor-reduced Matrigel and cultured with complete medium (control), HAGG (400 μg/mL), TNF-α (10 ng/mL), or TNF-α plus HAGG, respectively. Representative images of tube formation assay were captured after incubation for 12 h (A) and 20 h (B), using an inverted phase contrast microscope (magnification: 40 times). Quantifications of tube formation assays at 12 h (C) and 20 h (D), including the number of junctions and number of meshes, were evaluated by ImageJ software and plotted in column diagrams. Data were presented as mean ± SEM (n = 3). Comparisons between two groups were analyzed by paired t-test.

HAGG suppressed GEC tube formation on Matrigel. GECs were seeded on growth factor-reduced Matrigel and cultured with complete medium (control), HAGG (400 μg/mL), TNF-α (10 ng/mL), or TNF-α plus HAGG, respectively. Representative images of tube formation assay were captured after incubation for 12 h (A) and 20 h (B), using an inverted phase contrast microscope (magnification: 40 times). Quantifications of tube formation assays at 12 h (C) and 20 h (D), including the number of junctions and number of meshes, were evaluated by ImageJ software and plotted in column diagrams. Data were presented as mean ± SEM (n = 3). Comparisons between two groups were analyzed by paired t-test.