Table 1.

Primer and probe sequences used for the detection of Brucella genus and species.

Target	Primer/Probe	Target Gene	Sequence
Brucella genus	Forward	bcsp31	5′-GCTCGGTTGCCAATATCAATGC-3′
	Reverse		5′-GGGTAAAGCGTCGCCAGAAG-3′
	Probe		FAM-AAATCTTCCACCTTGCCCTTGCCATCA-BHQ1′
B. abortus	Forward	IS711	5′-GCGGCTTTTCTATCACGGTATTC-3′
	Reverse		5′-CATGCGCTATGATCTGGTTACG-3′
	Probe		FAM-CGCTCATGCTCGCCAGACTTCAATG-BHQ1
B. melitensis	Forward	IS711	5′-AACAAGCGGCACCCCTAAAA-3′
	Reverse		5′-CATGCGCTATGATCTGGTTACG-3′
	Probe		FAM-CAGGAGTGTTTCGGCTCAGAATAATCCACA-BHQ1