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. 2019 Oct 31;20(21):5434. doi: 10.3390/ijms20215434

Figure 4.

Figure 4

Immunofluorescent labelling of paraspeckle protein NONO in transfected healthy fibroblasts. (A) Immunolabelling of primary normal human fibroblasts following transfection with various 2′-Me PS AO lipoplexes at a concentration of 400 nM. Cells were fixed 24 hrs following transfection. Staining of the paraspeckle protein NONO shows sequestration and aggregation of NONO in various subcellular locations. (B) Immunofluorescent labelling of NONO following Endoporter transfection of primary normal human fibroblasts with various PMOs at concentrations of 10 or 5 μM. Cells were fixed 24 hrs following transfection. (C) Untreated (control) showing endogenous subcellular distribution of NONO. GTC = Gene Tools Control sequence. Scale bar is 20 μm.