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. Author manuscript; available in PMC: 2020 Oct 18.
Published in final edited form as: ACS Chem Biol. 2019 Sep 19;14(10):2176–2184. doi: 10.1021/acschembio.9b00381

Figure 1.

Figure 1.

(a) Titers of anti-MUC1 and anti-Qβ IgG antibodies from MUC1.Tg mice immunized with Qβ-MUC1 conjugates 16 and 17. For determination of anti-MUC1 IgG titers, the ELISA measurements were performed against the corresponding BSA-MUC1 conjugates 18 and 19. For testing levels of anti-Qβ IgG, the ELISA was performed against Qβ. Each symbol represents one mouse (n = 5 mice for each group). The titer was determined by regression analysis with log10 dilution plotted with optical density and reported as the highest fold of dilution giving the optical absorbance value of 0.1 over those of the preimmune control sera (OD = 0.2). IgG subtypes of MUC1.Tg mice immunized (b) Qβ-MUC1-STn 16 and (c) Qβ-MUC1-Tf 17 assayed against the corresponding BSA-MUC1 conjugates 18 and 19, respectively; ***p < 0.001. The p values were determined through a two-tailed unpaired Student’s t-test using GraphPad Prism.