KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-AID-tag	MBL	Cat# M214-3
Anti-PSTAIR	Sigma-Aldrich	Cat# P7962; RRID:AB_261183
Bacterial Strains and Plasmids
NEB 5-alpha Competent E. coli (High Efficiency)	New England Biolabs (NEB)	Cat# C29871
YIAL3-URA	(Pavlov et al., 2001)	N/A
P170-URA	(Clausen et al., 2015)	N/A
Biological Samples
Genomic DNA isolated from HeLa and HEK293T cells	Björn Rabe	N/A
Chemicals, Peptides, and Recombinant Proteins
Nocodazole	Sigma-Aldrich	Cat# M1404
Doxycycline hyclate	Sigma-Aldrich	Cat# D9891
3-indoleacetic acid (IAA)	Abcam	Cat# ab146403
Certified low melt agarose	Bio-rad	Cat# 1613111
SYBR Gold	Invitrogen	Cat# S11494
dCTP-alpha-P32 6000Ci/mmol	Perkin Elmer	Cat# BLU513H250
β Agarase I	NEB	Cat# M0392
Shrimp Alkaline Phosphatase	NEB	Cat# M0371
RNase HII	NEB	Cat# M0288
T4 RNA Ligase I	NEB	Cat# M0204
T7 DNA Polymerase	NEB	Cat# M0274
Zymolyase 20T	USBiological	Cat# 37340-57-1
Proteinase K	Roche	Cat# 03115852001
Critical Commercial Assays
MasterPure Yeast DNA Purification Kit	Lucigen	Cat# MPY80200
KAPA HiFi HotStart Ready Mix	Kapa Biosystems	Cat# KK2602
HighPrep PCR Clean-Up System	MagBio Genomics	Cat# AC-60050
Deposited Data
Agarose gels and Southern blot data	This paper	https://doi.org/10.17632/44hk5n646g.3
Raw and analyzed DNA sequencing data	This paper	https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE133558
Experimental Models: Organisms/Strains
S. cerevisiae: Strain background: W303 (Table S1)	R. Rothstein	N/A
Oligonucleotides (Table S2)
Software and Algorithms
Flow Jo	Flow Jo	https://www.flowjo.com/
ImageJ	ImageJ	https://imagej.nih.gov/ij/
Bowtie 2	(Langmead and Salzberg, 2012)	http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
Custom algorithm for HydEn-seq analysis	This paper
Other
CHEF-DR II Pulsed-Field Gel Electrophoresis system	Bio-rad	N/A