-l	The provided sgRNA library file, including the sgRNA id, the sequence, and the gene it is targeting (Table 2).
-n	The prefix of the output files.
--sample-label	The sample labels, separated by a comma (,). Must be equal to the number of samples provided (in --fastq option). Default “sample1, sample2, …”.
--fastq	The sample fastq files (or fastq.gz files), separated by a space; use a comma (,) to indicate technical replicates of the same sample.