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. 2019 Nov 19;13:56. doi: 10.1186/s40246-019-0243-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — EMSA of biotin-labeled oligonucleotides. Wild-type and variant oligonucleotides (30 bp) were designed and labeled with biotin for genetic variants identified in AMI patients, including g.28495805C>T(rs111458691), g.28495138G>C, and g.28494900G>A. EMSA was conducted with biotinylated oligonucleotides and the nuclear extracts from HEK-293 and H9c2 cells. Free probe was marked with an arrow at the bottom. The affected binding for transcription factors was marked with an open arrow