Table 4.
PCR primers for the VEGFR-1 gene promoter and 5′-UTR region
| PCR primers | Sequences | Location | Position | Products |
|---|---|---|---|---|
| Sequencing | ||||
| VEGFR-1-F1 | 5′-ACATCCCTCTGACGGGTTCCA-3′ | 28496221 | − 1092 bp | 770 bp |
| VEGFR-1-R1 | 5′-GTAAGCCGGGTGGAGGGAGT-3′ | 28495471 | − 338 bp | |
| VEGFR-1-F2 | 5′-GCCTCAGTCCTCCGTGCCAAGA-3′ | 28495608 | − 479 bp | 788 bp |
| VEGFR-1-R2 | 5′-ATGGTCAGCTACTGGGACACCGG-3′ | 28494821 | + 308 bp | |
| Functioning | ||||
| VEGFR-1-F | 5′-(KpnI)-GCTCCGTGCAGCCAGGACGA-3′ | 28,496,151 | − 1022 bp | 1307 bp |
| VEGFR-1-R | 5′-(HindIII)-GTGAGCGCGACGCGGCCT-3′ | 28,494,845 | + 284 bp | |
PCR primers are designed based on the genomic DNA sequence of the VEGFR-1 gene (NC_000013.11). The transcription start is at the position of 28495128 (+ 1). PCR polymerase chain reaction