Fig. 3.

The HCC microenvironment inhibits SIRT4 expression in TAMs, and SIRT4 silencing facilitates M2-like polarization. a-b Western blotting and qRT-PCR were employed to detect the protein level and mRNA level of SIRT4 in PMs treated with HCM at various concentrations and time intervals. c PMs were treated with the indicated dose of HCM for 24 h and then qRT-PCR was performed to assess the expression of Arg-1, CD206, and TNF-α. d Western blotting and qRT-PCR were utilized to detect the protein level and mRNA level of SIRT4 in PMs treated with LPS (100 ng/ml) for different time intervals. e-f PMs were transfected with shSIRT4-Lv or SIRT4-Lv with the indicated dose of HCM for 24 h and qPCR was performed to evaluate the expression of Arg-1, CD206, and TNF-α. g-h An ELISA array was performed to evaluate the expression of IL-10 and IL-12. * p < 0.05