Fig. 4.

SIRT4 interference modulates TAMs to M2-like polarization via the FAO-PPARδ-STAT3 signalling pathway. a and b The oxygen flux (respiration) in control, SIRT4KD and SIRT4OE TAMs. The OCR was measured under basal and oligomycin, FCCP and Rotenone/Antimycin-A treatment conditions. c qRT-PCR was used to detect the mRNA level of FAO genes. d qRT-PCR was used to detect the mRNA level of the mitochondrial gene. e Western blotting was used to detect the effect of SIRT4 silencing on p-STAT3 nuclear translocation in TAMs. f Retrovirally knocked down PPARδ by its inhibitor GSK3787 reversed the effects of shSIRT4-Lv on p-STAT3 nuclear translocation. g Retrovirally knocked down PPARδ reversed the effects of shSIRT4-Lv on the levels of phenotype markers in TAMs. h Retrovirally knocked down PPARδ reversed the effects of shSIRT4-Lv on IL-10 and IL-12 expression in TAMs. Statistical significance was calculated using Student’s t-test: *p < 0.05