Fig. 7.

JL5 enhances cell death induced by the Smac/DIABLO mimetic, AEG. a-c Cells were treated with JL5 and AEG alone and in combination for 48 h and cell counts performed. Studies represent the mean of 4 independent experiments. The Smac mimetic AEG40730 (AEG) by itself had no effect on cell death of the H1299 or A549 cells. JL5 overcame resistance to AEG in the H1299 cells but not the A549 cells. d Western blot analysis of H1299 cells treated for 48 h showed that the combination of JL5 and AEG caused a greater decrease in the expression of cIAP1 and XIAP. e In the A549 cells, AEG decreased cIAP1 expression but had no effect on the expression of XIAP. NS is a nonspecific band used as a loading control. f The A549 cells treated for 48 h were resistant to cell death induced by the combination of AEG, TRAIL, and JL5. g Western blot analysis demonstrates cells treated for 48 h with JL5 alone and together with AEG, activated caspase-3 as demonstrated by the production of 17 kDa and 19 kDa fragments. h Cell counts of H1299 cells treated for 48 h with JL5 and AEG with and without the pan-caspase inhibitor VAD. VAD significantly decreased cell death induced by JL5 in combination with AED, demonstrating cell death involved the activation of caspases. Studies represent the mean of 4 independent studies