Fig. 3.

DANCR targeted miR-135a-5p to regulate KLF8 expression in vitro. a Sequence alignments of DANCR with potential targeting sites of miR-135a-5p. b Luciferase reporter assay was performed to verify the binding effect between DANCR and miR-135a-5p. c–f Relative expression of miR-135a-5p was examined by qRT-PCR in CAL-27 cells (c), TCa-8113 cells (d), SCC9 cells (e) and TSCCA cells (f). g–j Relative expression of KLF8 was detected using qRT-PCR in CAL-27 cells (g), TCa-8113 cells (h), SCC9 cells (i) and TSCCA cells (j). ^$$$p < 0.001, versus to wt-DANCR + NC mimics. **p < 0.01, ***p < 0.001; ^&&&p < 0.001, versus to si-NC. ^{^^^}p < 0.001, versus to Vector