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. 2019 Nov 19;19:317. doi: 10.1186/s12906-019-2686-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Mice received different treatment in experiment. And IKK-α and iNOS expressions were detected by Western blot analysis into tissue homogenates from mice heart; GAPDH protein expression was used as loading control (a – c). Effect of DOX on NO release was evaluated by NO level in the serum of mice (d). (Means ± S.D., n = 10). *, significantly different (P < 0.05) from respective values in the control group;#, significantly different (P < 0.05) from respective values in the DOX group. Nitrotyrosine production in heart of ICR mice treat with DOX or SMI (e). Frozen myocardial tissue sections were stained with Anti-Nitrotyrosine (green) and nucleus with DAPI (blue) and were determined by immunofluorescence analysis. (Bar 200 μm)