Table 2.
The gradient changes of differentially expressed genes associated with lymph node metastasis or TNM stages with the Kruskal–Wallis test, and the survival analysis of patients with the differentially expressed genes
| Genes | Lymph node metastasis (N0–N1–N2) | TNM stages | Log-rank test | ||
|---|---|---|---|---|---|
| (I–II–III–IV) | |||||
| Gradient change | p | Gradient change | p | p | |
| NNAT | NA | 0.019 | NA | 0.025 | – |
| VSX2 | Yes, downtrend | 0.008 | – | 0.586 | 0.08025 |
| SCARNA7 | Yes, downtrend | 0.011 | Yes, downtrend | 0.018 | 0.34227 |
| AL161668.1 | NA | 0 | NA | 0.002 | – |
| SNORA12 | NA | 0.013 | NA | 0.003 | – |
| GSG1L2 | Yes, upward | 0 | – | 0.604 | 0.36278 |
| CYP2B6 | – | 0.287 | NA | 0.032 | – |
| ALB | – | 0.197 | NA | 0.008 | – |
| VN1R35P | Yes, upward | 0.003 | – | 0.157 | 0.08025 |
| SNORA71A | NA | 0.04 | – | 0.842 | – |
| AL451054.3 | NA | 0 | NA | 0.012 | – |
| AC105999.2 | Yes, upward | 0.042 | Yes, upward | 0.012 | 0.13752 |
| RN7SL3 | Yes, upward | 0.048 | – | 0.266 | 0.09487 |
| LINC01819 | Yes, downtrend | 0.016 | NA | 0.021 | – |
| RANBP20P | Yes, downtrend | 0.019 | Yes, downtrend | 0.016 | 0.07001 |
| RNU5A-1 | – | 0.066 | Yes, downtrend | 0.015 | 0.75953 |
| RN7SKP255 | – | 0.101 | NA | 0.005 | – |
| AL513304.1 | Yes, upward | 0.019 | – | 0.073 | 0.37489 |
| HIST1H4F | – | 0.191 | NA | 0 | – |
| RN7SKP203 | NA | 0.006 | – | 0.334 | – |
| HIST1H4L | – | 0.342 | NA | 0.048 | – |
| RN7SL769P | NA | 0.01 | – | 0.116 | – |
| RN7SL151P | Yes, downtrend | 0.006 | Yes, downtrend | 0.009 | 0.28316 |
| GKN1 | NA | 0.039 | – | 0.272 | – |
| FXNP2 | NA | 0.006 | – | 0.508 | – |
| RNY3 | NA | 0.003 | – | 0.067 | – |
| AC112495.1 | Yes, downtrend | 0.012 | NA | 0.002 | 0.88522 |
| SYNPR | Yes, downtrend | 0.034 | Yes, downtrend | 0.002 | 0.14163 |
| RN7SL480P | Yes, downtrend | 0.03 | – | 0.169 | 0.97413 |
| RN7SL116P | Yes, downtrend | 0.019 | – | 0.057 | 0.71102 |
| AC036111.1 | NA | 0.004 | – | 0.195 | |
| RNA5-8SP2 | NA | 0 | – | 0.088 | |
| RN7SL300P | NA | 0.026 | – | 0.079 | |
| HIST1H2AH | Yes, upward | 0.014 | NA | 0.012 | 0.89036 |
| PSG11 | – | 0.126 | NA | 0.002 | |
| GLRA4 | Yes, downtrend | 0.003 | – | 0.322 | 0.08082 |
| RN7SL359P | NA | 0 | – | 0.052 | – |
| AL135929.2 | NA | 0.006 | – | 0.14 | – |
| CYP11B1 | NA | 0.029 | – | 0.123 | – |
| RN7SL342P | NA | 0.02 | – | 0.062 | – |
| SPAG11B | Yes, upward | 0.028 | – | 0.064 | 0.54783 |
| RN7SL732P | NA | 0.005 | – | 0.082 | – |
| CYP1D1P | NA | 0 | NA | 0.002 | – |
| RN7SL791P | NA | 0 | NA | 0.002 | – |
| RN7SKP189 | NA | 0.002 | – | 0.696 | – |
| RN7SKP71 | Yes, downtrend | 0.011 | NA | 0.025 | 0.24259 |
| RN7SL217P | NA | 0.029 | NA | 0.041 | – |
| RN7SL272P | NA | 0 | NA | 0.016 | – |
| RHOXF2B | NA | 0 | – | 0.093 | – |
| RN7SL464P | NA | 0.003 | – | 0.214 | – |
| CRISP1 | NA | 0.007 | – | 0.074 | – |
| FGF4 | – | 0.379 | NA | 0.019 | – |
| CRP | NA | 0.026 | – | 0.066 | – |
| PSG2 | – | 0.347 | NA | 0.03 | – |
| RN7SL197P | NA | 0.017 | – | 0.644 | – |
| RN7SL646P | NA | 0.003 | – | 0.111 | – |
| RN7SL554P | NA | 0.001 | – | 0.317 | – |
| PPP1R3A | NA | 0.009 | – | 0.226 | – |
| RN7SL597P | – | 0.056 | NA | 0.017 | – |
| RN7SL308P | NA | 0.001 | NA | 0.003 | – |
| AC106872.1 | NA | 0 | NA | 0.003 | – |
| AL135929.1 | NA | 0.007 | – | 0.086 | – |
| AL512638.1 | Yes, upward | 0.002 | Yes, upward | 0 | 0.80925 |
| RN7SL711P | – | 0.104 | Yes, downtrend | 0.022 | 0.6968 |
| HMGB3P18 | NA | 0.018 | NA | 0.022 | – |
| RN7SL126P | NA | 0.021 | – | 0.106 | – |
| RN7SL630P | NA | 0.002 | – | 0.066 | – |
| RN7SL494P | Yes, downtrend | 0.025 | – | 0.057 | 0.02587 |
| RN7SL7P | NA | 0.024 | – | 0.23 | – |
| RN7SL786P | NA | 0.021 | – | 0.118 | – |
| AC108515.1 | NA | 0 | NA | 0.005 | – |
| RN7SKP185 | NA | 0.023 | Yes, downtrend | 0.02 | 0.66366 |
| RN7SKP90 | NA | 0 | Yes, downtrend | 0.017 | 0.91288 |
| AC008808.2 | – | 0.814 | NA | 0.024 | – |
| RN7SL390P | NA | 0.012 | – | 0.445 | – |
| SCARNA3 | NA | 0 | NA | 0.007 | – |
| MIR124-2HG | NA | 0.002 | NA | 0.012 | – |
| RN7SL297P | NA | 0.001 | NA | 0.002 | – |
| RNU1-88P | NA | 0.004 | – | 0.35 | – |
| RN7SL314P | NA | 0.078 | NA | 0.038 | – |
| RN7SL575P | NA | 0.049 | – | 0.272 | – |
| RN7SL302P | NA | 0.04 | – | 0.099 | – |
| AL513475.2 | NA | 0.046 | – | 0.401 | – |
| KRT38 | – | 0.148 | Yes, upward | 0.031 | 0.30421 |
| OR4A16 | NA | 0.004 | NA | 0.003 | – |
| FRG2 | NA | 0.003 | – | 0.699 | – |
| LINC02557 | NA | 0.001 | – | 0.462 | – |
| LINC01221 | NA | 0.002 | – | 0.076 | – |
| AC012065.1 | Yes, upward | 0 | NA | 0 | 0.25382 |
| LINC01040 | NA | 0.014 | NA | 0.024 | – |
| IGLV3-26 | NA | 0.003 | NA | 0.011 | – |
| CRCT1 | Yes, upward | 0.019 | NA | 0.013 | 0.51194 |
| GAGE12 J | NA | 0.017 | NA | 0.007 | – |
| CELA3A | Yes, downtrend | 0.035 | NA | 0.003 | 0.60893 |
| RN7SL260P | NA | 0.005 | – | 0.102 | – |
| AC245291.3 | – | 0.105 | NA | 0.018 | – |
| AC105031.2 | Yes, upward | 0.001 | NA | 0.013 | 0.88735 |
| AC245128.1 | NA | 0.008 | NA | 0.043 | – |
| AC008517.1 | NA | 0.002 | – | 0.357 | – |
| DRAXINP1 | – | 0.111 | NA | 0 | – |
| RN7SL14P | NA | 0.032 | – | 0.214 | – |
| DDX11L16 | NA | 0.002 | NA | 0.02 | – |
| ANHX | NA | 0.043 | NA | 0.007 | – |
| FAM9A | NA | 0.018 | NA | 0 | – |
| TMIGD1 | Yes, upward | 0.001 | Yes, upward | 0.027 | 0.42473 |
| PSG7 | – | 0.251 | Yes, upward | 0.001 | 0.74669 |
| AC105460.1 | NA | 0.01 | NA | 0.001 | – |
| AC080128.1 | – | 0.215 | NA | 0.036 | – |
| BX510359.3 | – | 0.064 | NA | 0.002 | – |
| AL139002.1 | NA | 0.022 | – | 0.747 | – |
| MIR3976HG | – | 0.195 | NA | 0.002 | – |
| SPAG11A | NA | 0.003 | NA | 0.008 | – |
d the deleted base, Pcorrected multiple testing by the Bonferroni correction, NA not applicable