Fig. 3.

Statistical results of the 2 pronuclear (2PN), 2-cell, and blastocyst stages of mouse oocytes after injection with either miR-451 inhibitor (n = 132) or negative control (NC) inhibitor (n = 95) and oocytes of the untreated control group (n = 245). The proportions of oocytes in the untreated control, NC inhibitor, and miR-451 inhibitor groups that developed into the 2PN stage (a) were 86.57% ± 7.15, 69.37% ± 7.48, and 35.12% ± 4.78%, respectively; into the 2-cell stage (b) were 78.59% ± 6.05, 61.63% ± 6.51, and 27.38% ± 5.31%, respectively; and into the blastocyst stage (c) were 59.43% ± 4.86, 41.26% ± 4.89, and 18.23% ± 2.32%, respectively. All experiments were repeated at least thrice. Unpaired t-test, *P < 0.05; **P < 0.01; ***P < 0.001. NC, negative control